Cell Biology & Bioimaging Core

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The Zeiss 510 Meta multiphoton cofocal microscope is currently capable of imaging both fixed and live cells on slides or glass-bottom petri dishes.  The instrument is equipped with the following:

• Zeiss Axiovert 200M
• Laser lines at 458, 477, 488, 514, 543 and 633 nm
• Coherent Chameleon pulsed infrared multiphoton tunable from 720-950 nm allows excitation of UV-excitable dyes as well as GFP and YFP
• Two PMTs, META detector and transmitted light detector for DIC imaging with fluorescent overlays
• Capable of time course experiments, Z-stack collection for 3D work, lambda stacks for spectral unmixing or emission spectral analysis (using META)
• FRET, FRAP, and FLIP as well as ratio imaging can be done
• Zeiss Incubator S system available for live cell experiments allowing humidity, CO2, and temperature control

• Objective list:
  • Fluar 10x/0.5 NA
  • Plan-Apochromat 20x/0.8 NA
  • Plan-Neofluar 40x/0.85 NA
  • Plan-Neofluar 40x/1.30 NA Oil
  • Plan Apochromat 63x/1.4 NA Oil
  • C-Apochromat 40x/1.2 NA Water is available

  • α-Plan Apochromat 100x/1.46 NA Oil is available

• Filter sets for:
• DAPI
• FITC
• CY3

• Zeiss 510 Operating Procedures (pdf format)

• Zeiss Brochure for LSM 510 NLO

• List of available filters for our Meta system

• FRET Macro Guide

• LSM 510 Macro Manual

• Guided tour for LSM 510 META

• LSM Image Browser Software