Cell Biology & Bioimaging Core

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The Everest digital microscopy workstation from Intelligent Imaging Innovations is based on a Zeiss Axioplan 2 upright microscope and is equipped with the following hardware and software components:

• Zeiss Axioplan 2 (brochure)
• Photometrics CoolSnap HQ CCD camera
• Applied Scientific Instruments MS-2000 XYZ stage with linear encoders
• Sutter Lambda LS 175W Xenon arc lamp and power supply
• Objective list:
  • Fluar 10x/0.5 NA
  • Plan Apochromat 20x/0.75 NA
  • Plan-Neofluar 40x/0.75 NA
  • Plan-Neofluar 40x/1.30 NA Oil
  • Plan Apochromat 63x/1.4 NA Oil
  • EC Plan Neofluar 2.5x/0.075 NA
  • Plan Apochromat 5x/0.16 NA
• Filter sets for:
  • DAPI (EX 360/40, EM 460/50, D 400dclp)
  • FITC (EX HQ487/25, EM GQ535/40, D Q505lp)
  • CY3 (EX HQ535/50, EM HQ610/75, D Q565lp)
  • TexasRed (EX HQ560/55, EM HQ645/75, D Q595lp)
• CY5 (EX HQ620/60, EM HQ700/75, D Q660lp)
• CY5.5 (EX HQ650/45, EM HQ710/50, D Q680lp)
• 3I's Slidebook Software v5.0 for image capture, data analysis, and 3D rendering

Click on one of the following links for a brief instruction manual on basic image capture with the Everest system - PDF format / Word Format